Biochemical oxygen demand BOD Test Kit
Estimation of BOD of water:
Reagents: Manganous sulphate solution
Alkail-iodine reagent
Starch indicator 0.00025 N Sodium thiosulphate solution Procedure:
. Three vials A, B and C was taken and filled with the water sample.
. Determine the DO of A immediately by adding 1 mL of MnSO4 + 2 mL of H2 SO4.
.ToBadding1mLofMnSO4 +2mLofH2 SO4andkeep for 5 days.
.ToCadding1mLofMnSO4 +2mLofH2 SO4 and2mL of sample water and keep for 5 days.
. After 5 days determine DO of B ( blank bottle) and C (sample bottle).
Calculation:
If the values of DO of the vials A, B and C be a, b and c then
BOD (mg /L) = (b-c) x 1000 / volume of sample in mL.
Result: The BOD of the sample is __________ mg /L.
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