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Ponceau S is a anionic dye commonly used for the detection of electro- blotted proteins after an electrophoretic separation prior to Western blotting [12–14] or MALDI-MS analysis [15].Ponceau S Staining Solution is supplied as ready to use. This product is recommended for rapid and reversible protein staining on nitrocellulose or PVDF membranes. This staining technique is often utilized to confirm protein electrotransfer in Western blotting assays prior to antibody-based detection.
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